Genomic RNA sequence of feline coronavirus strain FCoV C1Je
Identifieur interne : 003821 ( Main/Exploration ); précédent : 003820; suivant : 003822Genomic RNA sequence of feline coronavirus strain FCoV C1Je
Auteurs : Charlotte Dye [Royaume-Uni] ; Stuart G. Siddell [Royaume-Uni]Source :
- Journal of Feline Medicine and Surgery [ 1098-612X ] ; 2007.
Abstract
This paper reports the first genomic RNA sequence of a field strain feline coronavirus (FCoV). Viral RNA was isolated at post mortem from the jejunum and liver of a cat with feline infectious peritonitis (FIP). A consensus sequence of the jejunum-derived genomic RNA (FCoV C1Je) was determined from overlapping cDNA fragments produced by reverse transcriptase polymerase chain reaction (RT-PCR) amplification. RT-PCR products were sequenced by a reiterative sequencing strategy and the genomic RNA termini were determined using a rapid amplification of cDNA ends PCR strategy. The FCoV C1Je genome was found to be 29,255 nucleotides in length, excluding the poly(A) tail. Comparison of the FCoV C1Je genomic RNA sequence with that of the laboratory strain FCoV FIP virus (FIPV) 79-1146 showed that both viruses have a similar genome organisation and predictions made for the open reading frames and cis-acting elements of the FIPV 79-1146 genome hold true for FCoV C1Je. In addition, the sequence of the 3′-proximal third of the liver derived genomic RNA (FCoV C1Li), which encompasses the structural and accessory protein genes of the virus, was also determined. Comparisons of the enteric (jejunum) and non-enteric (liver) derived viral RNA sequences revealed 100% nucleotide identity, a finding that questions the well accepted ‘internal mutation theory’ of FIPV pathogenicity.
Url:
DOI: 10.1016/j.jfms.2006.12.002
Affiliations:
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Siddell</name><affiliation><wicri:noCountry code="syntax">???</wicri:noCountry></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>1 Department of Cellular and Molecular Medicine, Medical and Veterinary Sciences, University of Bristol, Bristol BS8 1TD</wicri:regionArea><wicri:noRegion>Bristol BS8 1TD</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Feline Medicine and Surgery</title><idno type="ISSN">1098-612X</idno><idno type="eISSN">1532-2750</idno><imprint><publisher>SAGE Publications</publisher><pubPlace>Sage UK: London, England</pubPlace><date type="published" when="2007">2007</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="202">202</biblScope><biblScope unit="page" to="213">213</biblScope></imprint><idno type="ISSN">1098-612X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1098-612X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">This paper reports the first genomic RNA sequence of a field strain feline coronavirus (FCoV). Viral RNA was isolated at post mortem from the jejunum and liver of a cat with feline infectious peritonitis (FIP). A consensus sequence of the jejunum-derived genomic RNA (FCoV C1Je) was determined from overlapping cDNA fragments produced by reverse transcriptase polymerase chain reaction (RT-PCR) amplification. RT-PCR products were sequenced by a reiterative sequencing strategy and the genomic RNA termini were determined using a rapid amplification of cDNA ends PCR strategy. The FCoV C1Je genome was found to be 29,255 nucleotides in length, excluding the poly(A) tail. Comparison of the FCoV C1Je genomic RNA sequence with that of the laboratory strain FCoV FIP virus (FIPV) 79-1146 showed that both viruses have a similar genome organisation and predictions made for the open reading frames and cis-acting elements of the FIPV 79-1146 genome hold true for FCoV C1Je. In addition, the sequence of the 3′-proximal third of the liver derived genomic RNA (FCoV C1Li), which encompasses the structural and accessory protein genes of the virus, was also determined. Comparisons of the enteric (jejunum) and non-enteric (liver) derived viral RNA sequences revealed 100% nucleotide identity, a finding that questions the well accepted ‘internal mutation theory’ of FIPV pathogenicity.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Dye, Charlotte" sort="Dye, Charlotte" uniqKey="Dye C" first="Charlotte" last="Dye">Charlotte Dye</name></noRegion><name sortKey="Siddell, Stuart G" sort="Siddell, Stuart G" uniqKey="Siddell S" first="Stuart G." last="Siddell">Stuart G. Siddell</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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